???????????????????SICM?????pH????????

???????????????????????Kanazawa University?????о???????????????????????о?????????????????????????ι????????????????Χ??pH??仯??????????????????????????????????????

??????????????????о?????Yuri Korchev??Yasufumi Takahashi??????????????Imperial College London????Yanjun Zhang????????????й??????????????????????????????????????????н??????“????????????????pH??????????????????????????????????????????á???????????????????????????????Χ??pH????????????????????????????в?????????????????????????????pH??仯??????????????????????”Zhang??Takahashi??Korchev??????·?????????????????????????????????????????ι?pH???????????????????0.01??λ???μ?pH??仯?????У????????2 ms??????????50 nm??

?о?????????????????????????ι??????Ч??????????е????????????????ι????????????????????????????Χ??pH?????????????????????????????????????Ч??譚???????????????????????????????????????????pH???仯??

Zhang??Takahashi??Korchev???????????????????????????????????????????????????????????????ι????????????????????????о?????????????????????????絼???????scanning ionic conductance microscope, SICM??????????????????

?????????????????в????????????????δ????????????????????????????м???????pH??????????????????????????У????????????????????μ???????????????pH??仯???????????????pH???????????????????????????????

?о??????????????????????????????????????pH????3D?????????????“???????????????”????????????????????????“?÷?????????????????????????????pHe???????pH????????????”

???÷??????????SICM-pH?????????????????????и?????3D pHe???????SICM?????????????????????A375M?????3D SICM???????????????3D pHe????????????????????????????pHe??????????????20 μm

??????????????

?????????pH????????繅?????????pH?о???pH???????Χ??????繅????????????????????????????仯????????????????????????????????衣?????????????????????????????????????????????????????????????????????????????????????

????????????ι?????????Ч????????о?????????????????м????????????????????????????????????Ч???????Ч????????????????????????????????ι???е?????????????????????

?????????

?????????????????????????????????????????????ι??е?????????????о???????????????poly-l-lysine, PLL???????????????glucose oxidase, GOx?????????????????г???????????????PLL???д??????????????Gox???д???????????л???????????????????????PLL/Gox???????

????pH?????￡???????????????д???????????????????????pH?????￡????簷???????????????λ??????????????????????????????????????????????

?????????https://www.nature.com/articles/s41467-019-13535-1

?????????

????????????-2019?桷

??????MEMS??????????????-2018?桷

???????????????????-2018?桷